spr SpectraMax Plus 384. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the emission at 460 nm over 1 h. Step 2. 3. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. Step 4. . Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. Twelve different automated tests are provided: • Fluorescence lower limit of detection (LLD) • Excitation wavelength accuracy • Emission wavelength accuracy • Excitation wavelength precisionHere, we present FluoroBrite™ DMEM, a cell culture media designed specifically for live-cell imaging that, when excited at wavelengths commonly used for FITC/GFP (i. Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. It may be installed in the top or bottom read detection cartridge transport. There were 12 replicate wells per dilution in both 96-well and 384- well plates. Catalog Number: M5. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Examples: “Press carefully to engage,” “ Do not press down. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. e. The. Consistent results are obtained over time offering workflow flexibility. Choose cables that meet the requirement described in this appendix. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Lmicroplate luminometer. SpectraMax M3, M4, and M5/M5e readers. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. With optimized reagents, option to operate the. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. 7" color, capacitive touch screen. Multimode Reader Comparison Chart. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . DOCUMENTATION. The M5 can read volumes down to 2uL with the. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). 00 USD. The chamber. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Description 1. 9000-0756. SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. • Tel. Access this by going to Help >. Multimode Reader Comparison Chart. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. High tolerance for contaminants. 1321N1, a human astrocytoma cell line, expresses. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. 0 out of 5 $ 17,999 $ 16,995. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. . D 1 1. Cavicide (Warehouse, Cat # 79300360) 6. SpectraMax M5e Multi-Mode Microplate Reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The absorbance and photo-luminescence was determined on a SpectraMax M5 plate reader via quartz cuvette using 1 × 10 10 particles per ml or lower and extrapolated when an optical density of 1 was. NORTH AMERICA. The dynamic range of the assay was approximately 5 orders of magnitude. Cavicide (Warehouse, Cat # 79300360) 6. . 0200-7027. 00 USD. 1. type Clear. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax M5 is a microplate reader that can be used for various applications, such as ELISA, Western blot, or cell culture. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. . The integration time suggested is 5-10 sec. For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. SoftMax Pro. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Customer. A wireless light sensor (701-999, PS-3212) was purchased from PASCO. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 5. Select a rugged, lightweight model designed to be used in the field. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. No filter change with dual monochromator optics. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. 005) Photometric PrecisionThe Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Five preferred detection modes. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. . l. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. Table of Contents. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. ISM Equipment Classification (Group 1, Class A) This equipment is. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. 2. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). Supplier: Molecular Devices. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. SoftMax Pro Software combines reader control and data analysis and provides sophisticated curve fitting and statistics for more in-depth bioassay analysis. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 40266 Email: drugdiscoverytech@lifetech. 2. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. 01) between the GFP-transfected and mock-transfected cells at an initial seeding. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. e. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Other technologies such as imaging. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. . LUMINESCENCE The SpectraMax M2 and SpectraMax M2e are microplate spectrofluorometers with photomultiplier tube detection. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. 6 lbs. Step 4: Incubate at room temperature for 1 hour, protected from light. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. These robust readers have been placed in labs from Antarctica to the. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Softmax Pro GxP Software 6. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-Mode We would like to show you a description here but the site won’t allow us. The . Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. Exhibit with us {{>currencyLabel}} Back. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. We would like to show you a description here but the site won’t allow us. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. In the M2, M5 and Plus 384, the light. The following document is intended to demonstrate setup of this instrument. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. Set the excitation at 531 nm and emission at 595 nm. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. 多功能酶标仪SpectraMax M5e. 4. Please Inquire. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. ability to detect 16 ng/well of DNA and to quantitate 50 ng of . . Offer Ends In: 18 Days 19: 38: 21. Add to Cart. 2. Cell viability was assessed by CellTiterGlo (Promega) reagent 96 hours after beginning treatment, and luminescence was measured on a Spectramax M5 spectrophotometer. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. 5. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. 190-1000. . the IMAP Platform and smoothly transition into high-throughput screening. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. edu) to be added as a trained user in iLab. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. UV/Vis absorbance and fluorescence spectra were measured on SpectraMax® M5 Multi- Mode Microplate Reader. Call 516-939-0888 to check current. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. . 2. TR-FRET assay. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax M5 and the Analyst GT. Note: In this user guide, all references to SpectraMax Multi-Mode. 40266 Email: [email protected]® M5多功能读板机为使用者提 供了除化学发光以外的其他检测方法，例 如：光吸收，荧光强度，荧光偏振和时间 分辨荧光。 SpectraMax® L和SpectraMax® M5酶标 仪均用SoftMax® Pro软件进行操控和设 置，该软件含有CellTiter-Glo检测模板和0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideExplore how the SpectraMax i3 Multi-Mode Platform provides an unlimited breadth of application possibilities, and how it can evolve with your future needs. The chamber. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. . After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Lower limits of detection for ATP determined using the ATPlite . Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. For example, microplates containing different samp les can be read using the same or Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Open the Softmax Pro software and select the FP option. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Plate chamber design of the SpectraMax category 1 microplate readers. The excitation and the emission wavelengths on. 1. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Cytokines in medium released. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The plate reader can measure multiple readouts, including UV and visible absorbance. Figure 2. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. 1 or greater versions. 95. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. Click SoftMax Pro Software User Guide. Step 2. Also refer to this Multimode Reader Comparison Chart. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. . Category 2 and 3 microplate readers use gradient heating as well. Table 1. Neutrophils from six HC patients were stimulated by PMA with or without pretreatment of SIRL-1 mAb, and then co-cultured with autologous monocytes for 12 h. Sale. View the Molecular Devices website. View regional contact information and Technical Support hours: Contact Us Hi Guest, welcome to. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. . From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. . 303 unable to cal dark current. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. UV/Vis Photometric Performance:. 314 low lamp level warning. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 4. 7°C and the bottom to 36. The chamber. . Read fluorescence polarization at room temperature (RT, 19–25°C). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Dual monochromators provide the ability for. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The following document is intended to demonstrate setup of this instrument. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. SpectraMax Spectrofluorometers. Molecular Devices SpectraMax M5 Multimode Microplate Reader. The hydrolysis was monitored continuously by top-read fluorescence using a Spectramax M5 multimode plate reader (Molecular Devices, USA) from the release of 7-amino-4-methylcoumarin (AMC) using an excitation wavelength of 380 nm and monitoring the. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Features. ” SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Sensitive fluorescent quantitation of DNA with the Quant-iT. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created DateThe Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. UV/Vis Photometric Performance:. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. Detection modalities are shown in Table 1-1. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Step 4: Incubate at room temperature for 1 hour, protected from light. Maximizes fluorescent signal from a wide range of cell types by reducing cell wash artifacts. SpectraMax DuoLuc Reporter Assay (SMP 6. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Luminescence was recorded in a Molecular Devices SpectraMax M5 plate reader using SoftMax Pro. . FlexStation® 3 Multi-Mode Microplate Reader. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Operated fromFluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. There were 12 replicate wells per dilution in both 96-well and 384- well plates. 316 grating motor driver faulty. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The PathCheck sensor can extend the instrument linearity by a factor of three or more. $4,499. 4. Read More. Read fluorescence polarization at room temperature (RT, 19–25°C). . 7°C (gradient heating design). General procedure forpreparedviscosity analysis The parent stock solution was prepared by dissolving probe FCV (5 mM) in DMSO. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 2 mm long, 86. . Page: 1/6. 0 out of 5 $ 15,999. For SpectraMax L or LMax II, LMax II. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). D 1. The SpectraMax M5 Multi-Mode. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. Description 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax M2 and SpectraMax M2 e are controlled by an external computer running SoftMax Pro software which provides integrat ed instrument control, data display, andSpectraMax M5 Multi-Mode Microplate Reader. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. Table of Contents. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. These lab workhorses are used in drug discovery, research, bioassay validation, and. . 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. Fluorescence intensity was measured using a. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). 306 plate air cal fail,low light. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Description. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Plate chamber design of the SpectraMax category 1 microplate readers. 72 MB. acquired on a SpectraMax M5 (Molecular Devices). The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. . Wash Buffer Concentrate was diluted 10-fold. com A. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Bottom-reading is usually recommended for cell-based assay with adherent cells. The M5 has two PMTs. 315 can't find zero order. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. . 4. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Molecular Devices. ) Click the "OK" button on SoftMax Pro Setup dialog box . Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. spr SpectraMax iD5 SpectraTest ABS2 v1. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. 1. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. The following document is intended to demonstrate setup of this instrument. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. 304 signal level saturation. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 0 out of 5 $ 5,999. edu) to be added as a trained user in iLab. The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. Formula accessors for reporting o The accessor!sectionname will return the name of the SoftMax Pro section containing the.